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Rat Tumor Necrosis Factor Alpha ELISA Kit (TNFa) (RK00029)

Publications (8) Datasheet

ABclonal:Rat Tumor Necrosis Factor Alpha ELISA Kit (TNFa)


Product nameRat Tumor Necrosis Factor Alpha ELISA Kit (TNFa)
Catalog No.RK00029
Size96 T
Sample100 ul
InstrumentMicroplate Reader
Assay Time4.0 hours
Assay TypeSandwich ELISA
Sample typeserum, plasma, cell culture supernates and other biological fluids
Cross reacts withRat
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, ).
Intended useThis Rat Tumor Necrosis Factor Alpha (TNFa) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat Tumor Necrosis Factor Alpha (TNFa) in the sample, this Rat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rat Tumor Necrosis Factor Alpha (TNFa) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore at 4°C
Sensitivity6.19 pg/mL
Standard curve range31.2-2000pg/ml
ComponentsMicrowell Plate coated with monoclonal antibody to Rat Tumor Necrosis Factor Alpha
Biotin-Conjugate anti-Rat Tumor Necrosis Factor Alpha antibody
Rat Tumor Necrosis Factor Alpha Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

* This package insert must be read in its entirety before using this product.

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