Product Type > Assay Kits > ELISA Kits > Rat ELISA Kits

Rat EGF ELISA Kit (RK00197)

Datasheet

ABclonal: - Rat EGF ELISA Kit (RK00197)

Overview

Product name Rat EGF ELISA Kit
Catalog No. RK00197
Size 96 T
Sample 100 ul
Instrument Microplate Reader
Detection Colorimetric
Assay Time 4 hours
Conjugate Biotin
Assay Type Sandwich ELISA
Sample type serum, plasma, cell culture supernates and other biological fluids
Cross reacts with Rat
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended use This Rat EGF ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat EGF in the sample, this Rat EGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rat EGF concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructions Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Sensitivity 3.9pg/mL
Standard curve range 7.8-500pg/mL
Components Microwell Plate coated with monoclonal antibody to Rat EGF
Biotin-Conjugate anti-Rat EGF antibody
Streptavidin-HRP
Rat EGF Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
Specification
Safety notes 1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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* For research use only. Not for therapeutic or diagnostic purposes.

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