Product Type > Assay Kits > ELISA Kits > ELISA Antibody Pairs

Rabbit anti-Human IL-6 mAb (DET) (RM17652)

ABclonal: - Rabbit anti-Human IL-6 mAb (DET) (RM17652)

Overview

Product name Rabbit anti-Human IL-6 mAb (DET)
Catalog No. RM17652
Catagory Elisa Antibody Kit
Application multiplex assay
Description Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates "classic signaling", whereas the binding of IL6 and soluble IL6R to IL6ST stimulates "trans-signaling". Alternatively, "cluster signaling" occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
Immunogen E. coli-derived human IL-6 protein
Pro29-Met212
Accession #Q75MH2
Purirty Affinity purification
Ig Type Rabbit IgG
Formulation Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1, PH 7.4.
CrossReactivity No cross-reactivity in multiplex assay with
Recombinant human CNTF、IL-2 、IL-3、G-CSF、 IL-4 、M-CSF、IL-5 、IL-1A、IL-1B 、IL-6RA、IL-6RB、IL-7、IL-8&Recombinant mouse IL-6、IL-2、IL-4、IL-11、 IL-12
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative:0.1%Braveds MB-1

Avoid repeated freeze-thaw cycles.

Application

Human IL-6 multiplex assay

 Recommended
Concentration
Sample
Capture10ug/106Rabbit anti-Human IL-6 mAb (CAP)(Cat. No. RM17650)
Detection0.5-2ug/mLRabbit anti-Human IL-6 mAb (DET)(Cat. No. RM17652)
Standard1.4-1000pg/mLRecombinant IL-6 Protein (Cat. No. RP00201)
ABclonal: - Rabbit anti-Human IL-6 mAb (DET) (RM17652)}

- Rabbit anti-Human IL-6 mAb (DET) (RM17652)

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

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