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Rabbit anti-Human IL-10 mAb (CAP) (RM17643)

ABclonal:

Overview

Product nameRabbit anti-Human IL-10 mAb (CAP)
Catalog No.RM17643
CatagoryPrimary antibody(CAP)
Applicationmultiplex assay
DescriptionMajor immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3.In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators.Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha.Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
ImmunogenRecombinant Human IL-10 Protein
Ser19-Asn178
Accession #P22301
PurirtyAffinity purification
Ig TypeRabbit IgG
Endotoxin Level< 1.0 EU/μg of the protein by LAL method.
FormulationSupplied as a 0.2um filtered solution in PBS, PH 7.4 containing Human IL-10 Antibody.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.

Avoid repeated freeze-thaw cycles.

Application

Human IL-10 multiplex assay

 Recommended
Concentration
Sample
ELISA Capture10ug/106Rabbit anti-Human IL-10 mAb (CAP)(Cat. No. RM17643)
ELISA Detection0.5-2ug/mLRabbit anti-Human IL-10 mAb (DET)(Cat. No. RM17644)
Standard1.4-1000pg/mLRecombinant IL-10 Protein (Cat. No. RM94521)
ABclonal:}

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

* For research use only. Not for therapeutic or diagnostic purposes.

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