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Mouse TNF-alpha ELISA Kit

Catalog No:RK00027
Size:96 T
Sample:100 ul
Instrument:Microplate Reader
Assay Time:4.0 hours
Precautions:Sandwich ELISA
ABclonal:Mouse TNF-alpha ELISA Kit

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RK00027Mouse TNF-alpha ELISA Kit (RK00027) suitable for serum, plasma, cell culture supernatants and urine in Mouse. 100% guaranteed. ABclonal provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for the competitive price.
PRODUCT OVERVIEWThe ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
INTENDED USEThis Mouse TNF-alpha ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Mouse TNF-alpha in the sample, this Mouse TNF-alpha ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Mouse TNF-alpha concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Standard Curve Range 31.25-2000 pg/mL
COMPONENTSMicrowell Plate coated with monoclonal antibody to mouse TNF
Biotin-Conjugate anti-mouse TNF antibody
Mouse TNF Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
SAFETY NOTES1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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