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Human Resistin ELISA Kit (RK00139)

Datasheet

ABclonal: - Human Resistin ELISA Kit (RK00139)

Overview

Product nameHuman Resistin ELISA Kit
Catalog No.RK00139
Size96 T
Sample100 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time4 hours
ConjugateBiotin
Assay TypeSandwich ELISA
Sample typeserum, plasma, cell culture supernates and other biological fluids
Cross reacts withHuman
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, ).
Intended useThis Human Resistin ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Resistin in the sample, this Human Resistin ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Resistin concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore unopened kit at 2-8 °C for1 week. If more than one week, please keep the components of the kit according to the instructions.Do not use past kit expiration date.
Sensitivity12.59pg/mL
Standard curve range31.2-2000pg/ml
ComponentsMicrowell Plate coated with monoclonal antibody to Human Resistin
Biotin-Conjugate anti-Human Resistin antibody
Streptavidin-HRP
Human Resistin Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
Specification
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

* This package insert must be read in its entirety before using this product.

* For research use only. Not for therapeutic or diagnostic purposes.

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Discontinued

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