Product Type > Assay Kits > ELISA Kits > Human ELISA Kits

Human Phosphoenolpyruvate carboxykinase (PCK) ELISA Kit (RK02027)

Datasheet

ABclonal: - Human Phosphoenolpyruvate carboxykinase (PCK) ELISA Kit (RK02027)

Overview

Product name Human Phosphoenolpyruvate carboxykinase (PCK) ELISA Kit
Catalog No. RK02027
Size 96 T
Sample 100 ul
Instrument Microplate Reader
Detection Colorimetric
Assay Time 4 hours
Conjugate Biotin
Assay Type sandwich ELISA
Sample type Serum, plasma and cell culture supernates.
Cross reacts with Human
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in serum, Plasma , Urine , tissue homogenates and Cell culture supernates and Other biological fluids
Intended use This Human Phosphoenolpyruvate carboxykinase (PCK) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Phosphoenolpyruvate carboxykinase (PCK) in the sample, this Human Phosphoenolpyruvate carboxykinase (PCK) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Phosphoenolpyruvate carboxykinase (PCK) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructions Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Sensitivity 0.1 ng/mL
Standard curve range 0.31-20 ng/mL
Components Microtiter Plate
Enzyme Conjugate
Standards
Substrate A
Substrate B
Stop Solution
Wash Solution
Balance Solution
Safety notes 1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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* For research use only. Not for therapeutic or diagnostic purposes.

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