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Human Epidermal Growth Factor Receptor ELISA Kit (EGFR) (RK01300)

Datasheet

ABclonal:Human Epidermal Growth Factor Receptor ELISA Kit (EGFR)

Overview

Product nameHuman Epidermal Growth Factor Receptor ELISA Kit (EGFR)
Catalog No.RK01300
Size96T
Sample100 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time3 hours
ConjugateHRP
PrecautionsSandwich ELISA
Sample typehuman serum, plasma, tissue homogenates and other biological fluids
Cross reacts withHuman
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended useThis Human Epidermal Growth Factor Receptor (EGFR) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Epidermal Growth Factor Receptor (EGFR) in the sample, this Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Epidermal Growth Factor Receptor (EGFR) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore at 4°C
Sensitivity1.22 ng/ml
Standard curve range3.12-200 ng/ml
ComponentsAntibody Coated Plate
Standard Lyophilized
Concentrated Biotin Conjugate Antibody
Streptavidin-HRP Concentrated
Standard/Sample Diluent
Biotin-Conjugate Antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer
TMB Substrate
Stop Solution
Plate Sealers
Specification
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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