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Human Collagen Type I Alpha 1 ELISA Kit (COL1a1) (RK01149)

Datasheet

ABclonal:

Overview

Product nameHuman Collagen Type I Alpha 1 ELISA Kit (COL1a1)
Catalog No.RK01149
Size96 T
Sample100 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time4.0 hours
ConjugateBiotin
Assay TypeSandwich ELISA
Sample typeserum, plasma and other biological fluids.
Cross reacts withHuman
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in serum, plasma and other biological fluids.
Intended useThis Human Collagen Type I Alpha 1 (COL1a1) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Collagen Type I Alpha 1 (COL1a1) in the sample, this Human Collagen Type I Alpha 1 (COL1a1) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Collagen Type I Alpha 1 (COL1a1) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore at 2-8℃ for 1 week
Sensitivity12.2pg/ml
Standard curve range31.2-2000pg/ml
ComponentsMicrotiter Plate
Enzyme Conjugate
Standards
Substrate A
Substrate B
Stop Solution
Wash Solution
Balance Solution
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

* This package insert must be read in its entirety before using this product.

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