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Human BAFF/BlyS/TNFSF13B ELISA Kit (RK00402)

Datasheet

ABclonal:Human BAFF/BlyS/TNFSF13B ELISA Kit

Overview

Product nameHuman BAFF/BlyS/TNFSF13B ELISA Kit
Catalog No.RK00402
Size96T
Sample100 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time4.0 hours
ConjugateHRP
PrecautionsSandwich ELISA
Sample typeserum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids
Cross reacts withHuman
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended useThis Human BAFF/BlyS/TNFSF13B ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human BAFF/BlyS/TNFSF13B in the sample, this Human BAFF/BlyS/TNFSF13B ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human BAFF/BlyS/TNFSF13B concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore at 4°C
Sensitivity28.51 pg/mL
Standard curve range62.5-4000 pg/mL
ComponentsMicrowell Plate coated with monoclonal antibody to Human BAFF/BlyS/TNFSF13B
Biotin-Conjugate anti-Human BAFF/BlyS/TNFSF13B antibody
Streptavidin-HRP
Human BAFF/BlyS/TNFSF13B Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
Specification
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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