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Human Apelin 17 ELISA Kit (AP17) (RK00909)

Datasheet

ABclonal:Human Apelin 17 ELISA Kit (AP17)

Overview

Product nameHuman Apelin 17 ELISA Kit (AP17)
Catalog No.RK00909
Size96T
Sample50 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time2 hours
ConjugateHRP
PrecautionsCompetition Inhibition ElISA
Sample typehuman serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Cross reacts withHuman
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended useThis Human Apelin 17 (AP17) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Apelin 17 (AP17) in the sample, this Human Apelin 17 (AP17) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Apelin 17 (AP17) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore at 4°C
Sensitivity9.14 pg/mL
Standard curve range24.69-2000 pg/mL
ComponentsAntibody Coated Plate
Standard Lyophilized
Concentrated Biotin Conjugate Antigen
Streptavidin-HRP Concentrated
Standard/Sample Diluent
Biotin Conjugate Antigen Diluent
Streptavidin-HRP Diluent
Wash Buffer
TMB Substrate
Stop Solution
Plate Sealers
Specification
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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