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General 12-Hydroxyeicosatetraenoic Acid ELISA Kit (12-HETE) (RK00603)

Datasheet

ABclonal:General 12-Hydroxyeicosatetraenoic Acid ELISA Kit (12-HETE)

Overview

Product nameGeneral 12-Hydroxyeicosatetraenoic Acid ELISA Kit (12-HETE)
Catalog No.RK00603
Size96T
Sample50 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time2 hours
ConjugateHRP
PrecautionsCompetition Inhibition ElISA
Sample typeserum, plasma and other biological fluids
Cross reacts withGeneral
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended useThis General 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of General 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample, this General 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus General 12-Hydroxyeicosatetraenoic Acid (12-HETE) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructionsStore at 4°C
Sensitivity5.32 ng/ml
Standard curve range12.35-1000 ng/ml
ComponentsAntibody Coated Plate
Standard Lyophilized
Concentrated Biotin Conjugate Antigen Lyophilized
Streptavidin-HRP Concentrated
Biotin-Conjugate Antigen reagent
Standard/Sample Diluent
Biotin Conjugate Antigen Diluent
Streptavidin-HRP Diluent
Wash Buffer(30x)
TMB Substrate
Stop Solution
Plate Sealers
Specification
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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