|Product name||Recombinant Human CD40 Ligand/CD154/CD40L/TNFSF5 Protein|
|Description||Recombinant Human CD40 Ligand/CD154/CD40L/TNFSF5 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Met113-Leu261) of human CD40 Ligand/CD154/CD40L/TNFSF5 (Accession #P29965).|
|Bio-Activity||Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The ED50 for this effect is 1-3 μg/mL in the presence of 20 ng/mL of recombinant human IL-4.|
|Synonym||CD154; CD40L; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP|
|Expressed Host||Human cells|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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