|Product name||Recombinant Human CD27/TNFRSF7 Protein|
|Description||Recombinant Human CD27/TNFRSF7 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr21-Ile192) of human CD27/TNFRSF7 (Accession #P26842) fused with an Fc, 6×His tag at the C-terminus.|
|Bio-Activity||Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED50 for this effect is 0.75-3 μg/mL in the presence of 10 μg/mL (100 μL/well) Recombinant Human CD27/TNFRSF7 and anti-CD3.|
|Synonym||S152; S152.LPFS2; T14; TNFRSF7; Tp55|
|Expressed Host||Human cells|
|Tag||Fc, 6×His tag at the C-terminus|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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