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Recombinant Human CD27/TNFRSF7 Protein (RP00326)

Datasheet

Overview

Product nameRecombinant Human CD27/TNFRSF7 Protein
Catalog No.RP00326
DescriptionRecombinant Human CD27/TNFRSF7 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr21-Ile192) of human CD27/TNFRSF7 (Accession #P26842) fused with an Fc, 6×His tag at the C-terminus.
Bio-ActivityMeasured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED50 for this effect is 0.75-3 μg/mL in the presence of 10 μg/mL (100 μL/well) Recombinant Human CD27/TNFRSF7 and anti-CD3.
Purity> 95% by SDS-PAGE.
Endotoxin< 1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
SpeciesHuman
This protein is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
SynonymS152; S152.LPFS2; T14; TNFRSF7; Tp55
Expressed HostHuman cells
TagFc, 6×His tag at the C-terminus
Swiss portP26842
Gene ID939
StorageStore the lyophilized protein at -20°C to -80 °C for long term.|After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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