Product Type > Proteins

Recombinant Human CD14 Protein (RP00323)

Datasheet

Overview

Product name Recombinant Human CD14 Protein
Catalog No. RP00323
Description Recombinant Human CD14 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr20-Cys352) of human CD14 (Accession #P08571) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.75-4.5 ng/mL.
Purity > 95% by SDS-PAGE.
Endotoxin < 1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.Contact us for customized product form or formulation.
Species Human
This protein is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
Synonym CD14
Expressed Host HEK293 cells
Tag C-6×His
Swiss-Prot P08571
Gene ID 929
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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