Product Type > Proteins

Recombinant Streptococcus pyogenes M1 Cas9 Protein (RP02063)

Datasheet

Overview

Product name Recombinant Streptococcus pyogenes M1 Cas9 Protein
Catalog No. RP02063
Description Recombinant Streptococcus pyogenes M1 CRISPR-Cas9 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Asp1368) of Streptococcus pyogenes M1 CRISPR-Cas9 (Accession #WP_010922251.1) fused with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.
Purity > 90 % as determined by SDS-PAGE.
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Formulation Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.
Species Streptococcus pyogenes M1
Calculated MW 163kDa
Synonym CRISPR-Cas9; Cas9
Expressed Host Baculovirus-Infected Sf9 Cells
Tag C-His
Swiss-Prot Q99ZW2
Gene ID 69900935
Storage This product is stable at ≤ ‑70°C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

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* For research use only. Not for therapeutic or diagnostic purposes.

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