Product name | Recombinant SARS-CoV-2 Spike S1 Protein |
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Catalog No. | RP01280 |
Description | Recombinant SARS-CoV-2 Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of sars-cov-2 Spike S1 (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus. |
Bio-Activity |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2-His Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike S1-mFc Protein, the EC50 of Recombinant SARS-CoV-2 Spike S1-mFc Protein is 79.21 ng/mL. |
Purity | > 85% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | SARS-CoV-2 |
Synonym | Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein |
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Expressed Host | HEK293 cells |
Tag | C-mFc |
Swiss-Prot | P0DTC2 |
Gene ID | 43740568 |
Storage | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. or This liquid product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.