Active Recombinant SARS-CoV-2 Spike RBD Protein (RP01258)

Product name	Active Recombinant SARS-CoV-2 Spike RBD Protein
Catalog No.	RP01258
Description	Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike RBD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. 2.Immobilized human ACE2 on COOH Chip can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR).
Purity	>95% by SDS-PAGE;> 95% by HPLC
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Species	SARS-CoV-2
Calculated MW	25.9kDa

Synonym	S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein
Expressed Host	HEK293 cells
Tag	C-His
Gene ID	43740568

Storage	Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Reconstitution	For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.