|Product name||Recombinant Mouse IL-4 Protein|
|Description||Recombinant Mouse IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His23-Ser140) of mouse IL-4 (Accession #NP_067258.1) fused with a 6×His tag at the C-terminus.|
1.Measured in a cell proliferation assay using MC/9 2 mouse mast cells. The ED50 for this effect is 2.8-11.3 pg/mL, corresponding to a specific activity of 8.85×107~3.57×108 units/mg.
2.Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.21-0.85 ng/mL, corresponding to a specific activity of 1.18×106~4.76×106 units/mg.
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.|
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* For research use only. Not for therapeutic or diagnostic purposes.