Product name | Recombinant Human TNF-alpha Protein |
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Catalog No. | RP00001 |
Description | Recombinant Human TNF-alpha Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val77-Leu233) of human TNF-alpha (Accession #NP_000585.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus. |
Bio-Activity |
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 2.4-9.6 pg/mL, corresponding to a specific activity of 1.04×108~4.16×108 units/mg. |
Purity | > 97% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.