|Product name||Recombinant Human TNF-alpha Protein|
|Description||Recombinant Human TNF-alpha Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val77-Leu233) of human TNF-alpha (Accession #NP_000585.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.|
|Bio-Activity||Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/mL.|
|Purity||> 97% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.|
|Synonym||DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F|
|Expressed Host||E. coli|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
* For research use only. Not for therapeutic or diagnostic purposes.
Submit your question about RP00001 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.