Product Type > Proteins

Recombinant Human Klotho beta/KLB Protein (RP02210)

Datasheet

Overview

Product name Recombinant Human Klotho beta/KLB Protein
Catalog No. RP02210
Description Recombinant Human Klotho beta/KLB Protein is produced by mammalian expression system. The target protein is expressed with sequence (Met30-Thr983) of human Beta Klotho (Accession #) fused with a His tag at the C-terminus.
Bio-Activity Immobilized Human Klotho at 1 μg/mL(100 μL/Well).Dose response curve for Anti-Klotho Ab.1 with the EC50 of 0.4 μg/ml determined by ELISA.
Immobilized Human Klotho at 2μg/ml(100 μL/Well).Dose response curve for Anti-Klotho Ab. 2 with the EC50 of 20.5 ng/mL determined by ELISA.
Purity > 95% as determined by Tris-Bis PAGE.> 95% as determined by HPLC
Endotoxin < 0.5EU per μg by the LAL method.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Species Human
Klotho is an anti-aging single-pass membrane protein predominantly produced in the kidney, with shedding of the amino-terminal extracellular domain into the systemic circulation. Circulating levels of soluble Klotho decrease with age, and the klotho gene is associated with increased risk of age-related diseases.
Synonym KLB; BKL
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot Q86Z14
Gene ID 152831
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Reconstitution Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Inquire About This Product

Submit your question about RP02210 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Antibodies (1)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Related Products

Antibodies (1)

Contact us to order