Product name | Recombinant Human Klotho beta/KLB Protein |
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Catalog No. | RP02210 |
Description | Recombinant Human Klotho beta/KLB Protein is produced by mammalian expression system. The target protein is expressed with sequence (Met30-Thr983) of human Beta Klotho (Accession #) fused with a His tag at the C-terminus. |
Bio-Activity |
Immobilized Human Klotho at 1 μg/mL(100 μL/Well).Dose response curve for Anti-Klotho Ab.1 with the EC50 of 0.4 μg/ml determined by ELISA. Immobilized Human Klotho at 2μg/ml(100 μL/Well).Dose response curve for Anti-Klotho Ab. 2 with the EC50 of 20.5 ng/mL determined by ELISA. |
Purity | > 95% as determined by Tris-Bis PAGE.> 95% as determined by HPLC |
Endotoxin | < 0.5EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.