Product Type > Proteins

Recombinant Human IgG2A Protein (RPT0005)

Datasheet

Overview

Product name Recombinant Human IgG2A Protein
Catalog No. RPT0005
Description Recombinant Human IgG2A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu99-Lys326) of human IgG2A (Accession #) fused with and NO-tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA.Immobilized Human CD32a (Catalog: RP00081) at 2 μg/mL (100 μL/well) can bind Human IgG2 (Catalog: RPT0005) with a linear range of 1.2-338 ng/mL.
Purity > 97% by SDS-PAGE.
Endotoxin <0.1EU/μg
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species Human
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens
Synonym IGHG2; Immunoglobulin heavy constant gamma 2; IgG2
Expressed Host HEK293 cells
Tag NO-tag
Swiss-Prot P01859
Gene ID 3501
Storage Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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