Product name | Recombinant Human IgG2A Protein |
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Catalog No. | RPT0005 |
Description | Recombinant Human IgG2A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu99-Lys326) of human IgG2A (Accession #) fused with and NO-tag at the C-terminus. |
Bio-Activity |
Measured by its binding ability in a functional ELISA.Immobilized Human CD32a (Catalog: RP00081) at 2 μg/mL (100 μL/well) can bind Human IgG2 (Catalog: RPT0005) with a linear range of 1.2-338 ng/mL. |
Purity | > 97% by SDS-PAGE. |
Endotoxin | <0.1EU/μg |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80°C for 12 months. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.