Product Type > Proteins

Active Recombinant Human IL-4 Protein (RP00995)

Publication (1) Datasheet COA

Overview

Product name Active Recombinant Human IL-4 Protein
Catalog No. RP00995
Description Active Recombinant Human IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 85-340 pg/mL, corresponding to a specific activity of 2.94 × 106~1.17× 107 units/mg.
Purity > 95% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.
Synonym BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4; BCGF1; BSF-1; BSF1; IL-4
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot P05112-1
Gene ID 3565
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Inquire About This Product

Submit your question about RP00995 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using RP00995? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order