|Product name||Recombinant Human IL-15 Protein|
|Description||Recombinant Human IL-15 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn49-Ser162) of human IL-15 (Accession #NP_000576.1) fused with a Fc, 6×His tag at the C-terminus.|
1.Measured in a cell proliferation assay using HT-2 cells. The ED50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 1.25 × 105~5.0× 105 units/mg.
2.Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 24.29-97.14 ng/mL, corresponding to a specific activity of 1.03 × 104~4.12× 104 units/mg.
|Purity||>95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4. Contact us for customized product form or formulation.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.|
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* For research use only. Not for therapeutic or diagnostic purposes.