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Active Recombinant Human IL-2-RA/CD25 Protein (RP00076)

Datasheet

Overview

Product nameActive Recombinant Human IL-2-RA/CD25 Protein
Catalog No.RP00076
DescriptionActive Recombinant Human IL-2-RA/CD25 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu22-Cys213) of human CD25/IL-2R alpha (Accession #NP_000408.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Recombinant human IL2R at 2 μg/mL (100 μL/well) can bind Recombinant human IL2 with a linear range of 5-30 ng/mL.
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.
SynonymCD25; IDDM10; IL2R; IMD41; p55; TCGFR
Expressed HostHEK293 cells
TagC-His
Swiss-ProtP01589
Gene ID3559
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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