Product Type > Proteins

Recombinant Rat CD73/5'-Nucleotidase/NT5E Protein (RP00570)

Datasheet

Overview

Product name Recombinant Rat CD73/5'-Nucleotidase/NT5E Protein
Catalog No. RP00570
Description Recombinant Rat CD73/5'-Nucleotidase/NT5E Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Trp29-Lys549) of rat CD73/5'-Nucleotidase/NT5E (Accession #P21588) fused with a 6×His tag at the C-terminus.
Purity > 90% by SDS-PAGE.
Endotoxin < 1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.
Species Rat
CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosylphophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymesresponsible for the production of extracellular adenosine, a signaling molecule that is involved in responses toinflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeablenucleosides.
Synonym CD73; E5NT; Nucleotidase; NT5E; ecto-5'-nucleotidase
Expressed Host HEK293 cells
Tag C-6×His
Swiss-Prot P21588
Gene ID 58813
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Inquire About This Product

Submit your question about RP00570 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order