Product Type > Proteins

Recombinant Mouse Sonic hedgehog protein/SHH Protein (RP00632)

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Overview

Product name Recombinant Mouse Sonic hedgehog protein/SHH Protein
Catalog No. RP00632
Description Recombinant Mouse Sonic hedgehog protein/SHH Protein is produced by E. coli expression system. The target protein is expressed with sequence (Cys25-Gly198) of mouse Shh/Sonic Hedgehog (Accession #Q62226) fused with an initial Met at the N-terminus.
Purity > 95% by SDS-PAGE.
Endotoxin < 1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.Contact us for customized product form or formulation.
Species Mouse
Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other twofamily members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signalingmolecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles inregulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shhis synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. Themature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment isthe core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat andgerbil Shh-N, respectively.
Synonym 9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081; SHH
Expressed Host E. coli
Tag No tag
Swiss-Prot Q62226
Gene ID 20423
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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