Overview
Product name |
Recombinant Mouse IgG2A Fc Protein |
Catalog No. |
RP00802 |
Description |
Recombinant Mouse IgG2A Fc Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Pro99-Lys330) of mouse IgG2A Fc (Accession #P01863). |
Purity |
> 95% by SDS-PAGE. |
Endotoxin |
< 1 EU/μg of the protein by LAL method. |
Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.Contact us for customized product form or formulation. |
Species |
Mouse |
Background
As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotypethat can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, andconstitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens byagglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked bydisulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fabfragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum amongthe four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragmentis demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A onthe surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and hasnumerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested torepresent a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Product information
Synonym |
Ig gamma-2 chain C region; IgG2A Fc |
Expressed Host |
HEK293 cells |
Tag |
No tag |
Swiss-Prot |
P01863 |
Usage guide
Storage |
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
Reconstitution |
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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* For research use only. Not for therapeutic or diagnostic
purposes.