Product Type > Proteins

Recombinant Mouse FGF-1/aFGF Protein (RP00487)

Datasheet

Overview

Product name Recombinant Mouse FGF-1/aFGF Protein
Catalog No. RP00487
Description Recombinant Mouse FGF-1/aFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Phe16-Asp155) of mouse FGF1/FGFa/FGF acidic (Accession #P61148).
Purity > 95% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, pH7.5, 50 mM Na2SO4, 0.5 mM DTT, 1 mM EDTA. Contact us for customized product form or formulation.
Species Mouse
FGF acidic is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which isproduced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells ofneuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF-acidic is a non-glycosylated heparin binding growth factor that isexpressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes andendothelial cells. FGF-acidic has the ability to signal through all the FGF receptors.
Synonym Fibroblast Growth Factor 1; FGF-1; Acidic Fibroblast Growth Factor; aFGF; Heparin-BindingGrowth Factor 1; HBGF-1; Fgf1; Fgf-1; Fgfa
Expressed Host E. coli
Tag No tag
Swiss-Prot P61148
Gene ID 14164
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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