|Product name||Recombinant Human VEGFA 121 Protein|
|Description||Recombinant Human VEGFA 121 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala207-Arg327 (Lys321Asn)) of human VEGFA 121 (Accession #NP_001020541.2).|
|Bio-Activity||Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard.|
|Synonym||MVCD1; VEGF; VPF|
|Expressed Host||E. coli|
|Purity||> 97% by SDS-PAGE.|
|Endotoxin||< 1.0 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 100mM NaCl, pH 8.0.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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