|Product name||Recombinant Human TNF-alpha Protein|
|Description||Recombinant Human TNF-alpha Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val77-Leu233) of human TNF-alpha (Accession #NP_000585.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.|
|AA Sequence ||VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL|
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. KNockout studies in mice also suggested the neuroprotective function of this cytokine.
|Synonym||DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F|
|Expressed Host||E. coli|
|Tag||6×His tag at the C-terminus|
|Purity||> 97% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method. |
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.
|After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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