Product Type > Proteins

Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) Protein (RP00056)

Datasheet

ABclonal:Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) Protein

Overview

Product name Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) Protein
Catalog No. RP00056
Description Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Cys24-Gly197 (Cys24Ile-Val-Ile)) of human Sonic hedgehog N-product (Accession #NP_000184.1).
Bio-Activity Measured by its ability to inhibit p53 expression in C3H10T1/2 mouse embryonic fibroblast cells. 1.25-2.5 μg/mL of Recombinant Human Sonic hedgehog can effectively decrease p53 expression.
Purity > 97% by SDS-PAGE.
Endotoxin < 1.0 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 300mM NaCl, pH 7.4.Contact us for customized product form or formulation.
Species Human
This protein is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Synonym HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; SHH; HHG1; sonic hedgehog; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Expressed Host E. coli
Tag No tag
Swiss-Prot Q15465
Gene ID 6469
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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