|Product name||Recombinant Human PD-1 Protein|
|Description||Recombinant Human PD-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu25-Thr168) of human PD-1 (Accession #NP_005009.2) fused with a 6×His tag at the C-terminus.|
|Bio-Activity||Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human B7-H1/PD-L1 Fc Chimera that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.|
|Synonym||CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2|
|Expressed Host||HEK293 cells|
|Tag||6×His tag at the C-terminus|
|Purity||> 97% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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