|Product name||Recombinant Human MANF Protein|
|Description||Recombinant Human MANF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Leu25-Leu182) of human MANF (Accession #NP_006001.4).|
|Bio-Activity||Measured by its ability to support the survival and stimulate neurite outgrowth of rat embryonic cortical neurons. The ED50 for this effect is 0.7-2.8 μg/mL.|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Expressed Host||E. coli|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term. |After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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