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Recombinant Human IL6 Protein (RP00004)

Datasheet

Overview

Product nameRecombinant Human IL6 Protein
Catalog No.RP00004
DescriptionRecombinant Human IL6 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro29-Met212) of human IL6 (Accession #NP_000591.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
Bio-ActivityMeasured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 x 105 IU/μg.
Swiss portP05231
Gene ID3569
SpeciesHuman
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. The encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this protein is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
SynonymBSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Expressed HostE. coli
Tag6×His tag at the C-terminus
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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