Product Type > Proteins

Recombinant Human IL-33 Protein (RP00499)

Datasheet

Overview

Product name Recombinant Human IL-33 Protein
Catalog No. RP00499
Description Recombinant Human IL-33 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser112-Thr270) of human IL-33 (Accession #O95760) fused with an initial Met at the N-terminus.
Purity > 95% by SDS-PAGE.
Endotoxin < 1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 7.5.Contact us for customized product form or formulation.
Species Human
Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in highendothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities anda chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in humantonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs)and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitropolarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severepathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.
Synonym IL33; C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV
Expressed Host E. coli
Tag No tag
Swiss-Prot O95760
Gene ID 90865
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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