|Product name||Recombinant Human IL-1 beta Protein|
|Description||Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.|
|Bio-Activity||Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is <12 pg/mL. The specific activity of recombinant human IL-1 beta is approximately 1.4 x 105 IU/μg, which is calibrated against recombinant human IL-1 beta WHO International Standard.|
|Synonym||IL-1; IL1-BETA; IL1F2|
|Expressed Host||E. coli|
|Tag||6×His tag at the C-terminus|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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