Product Type > Proteins

Recombinant Human IL-1 beta Protein (RP00002)

Datasheet COA

ABclonal:Recombinant Human IL-1 beta Protein

Overview

Product name Recombinant Human IL-1 beta Protein
Catalog No. RP00002
Description Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
Bio-Activity 1.Recombinant Human IL1β protein was measured by NF-κB reporter assay in HEK293 cells. The ED50 for this effect is 1-10 pg/mL, corresponding to a specific activity of 1×108~1×109 units/mg.
2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.217-0.870 ng/mL, corresponding to a specific activity of 1.15×106~4.61×106 units/mg.
3.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL.
Purity > 95% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Synonym IL-1; IL1-BETA; IL1F2; IL1 beta; IL1B
Expressed Host E. coli
Tag C-His
Swiss-Prot P01584
Gene ID 3553
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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