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Recombinant Human IFNA1 Protein (RP00011)

Datasheet

Overview

Product nameRecombinant Human IFNA1 Protein
Catalog No.RP00011
DescriptionRecombinant Human IFNA1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Cys24-Glu189 (Gln114Ala)) of human IFNA1 (Accession # NP_076918.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
Bio-ActivityMeasured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is 40-200pg/mL.
Swiss portP01562
Gene ID3439
SpeciesHuman
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells.Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has has both anti-viral and immunomodulatory activities on target cells.
SynonymIFL; IFN; IFN-ALPHA; IFN-alphaD; IFNA13; IFNA@
Expressed HostE. coli
Tag6×His tag at the C-terminus
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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