|Product name||Recombinant Human HMGB1 Protein|
|Description||Recombinant Human HMGB1 Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Met1-Glu215) of human HMGB1 (Accession #NP_002119.1).|
|Bio-Activity||Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is typically 20-100μg/ml.|
High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity. HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide. HMGB1 binds DNA in a non-sequence specific manner and may act as a structural cofactor during gene transcription.Acetylation of HMGB1 results in its cytoplasmic localization and eventual secretion. HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis.
|Synonym||HMG-1; HMG1; HMG3; SBP-1|
|Expressed Host||Baculovirus-Infected Sf9 Cells|
|Purity||> 97% by SDS-PAGE.|
|Endotoxin||< 1.0 EU/μg of the protein by LAL method. |
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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