|Product name||Recombinant Human Galectin-1 Protein|
|Description||Recombinant Human Galectin-1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Asp135) of human Galectin-1 (Accession #NP_002296.1).|
|Bio-Activity||Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.5-3 μg/ml.|
Galectin-1, also known as LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, Non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted by non-classical pathways. Secreted Galectin-1 has immunosuppressive and anti-inflammatory properties and suppresses acute and chronic inflammation and autoimmunity. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation. Galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.
|Expressed Host||E. coli|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method. |
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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