Product Type > Proteins

Recombinant Human FGF-12 Protein (RP00064)

Datasheet

Overview

Product name Recombinant Human FGF-12 Protein
Catalog No. RP00064
Description Recombinant Human FGF-12 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr181) of human FGF-12 (Accession #NP_004104.3) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR4 at 5 μg/mL (100 μL/well) can bind recombinant human FGF12 with a linear range of 35-100 ng/mL.
Purity > 97% by SDS-PAGE.
Endotoxin < 1.0 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, 1mM DTT, 300mM NaCl, pH 7.4.Contact us for customized product form or formulation.
Species Human
The protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted.
Synonym FGF12; EIEE47; FGF12B; FHF1
Expressed Host E. coli
Tag C-His
Swiss-Prot P61328
Gene ID 2257
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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