Product Type > Proteins

Recombinant Human CD28/TP44 Protein (RP00601)

Datasheet

Overview

Product name Recombinant Human CD28/TP44 Protein
Catalog No. RP00601
Description Recombinant Human CD28/TP44 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Asn19-Pro152) of human CD28/TP44 (Accession #P10747) fused with an Fc tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. When Recombinant Human CD28/TP44 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Purity > 95% by SDS-PAGE.
Endotoxin < 1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.
Species Human
T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one ofthe molecules expressed on T cells that provide co-stimulatory signals, which are required for T cellactivation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86(B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presentingcells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptorconstitutively expressed on naive T cells.
Synonym CD28; Tp44
Expressed Host HEK293 cells
Tag C-Fc
Swiss-Prot P10747
Gene ID 940
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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