|Product name||Recombinant Human B7-1/CD80 Protein|
|Description||Recombinant Human B7-1/CD80 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Val35-Asn242) of human B7-1/CD80 (Accession #NP_005182.1) fused with a 6×His tag at the C-terminus.|
|Bio-Activity||Measured by its binding ability in a functional ELISA. When Recombinant Human B7-1/CD80 is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CTLA-4 Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.|
|Synonym||B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7|
|Expressed Host||HEK293 cells|
|Tag||6×His tag at the C-terminus|
|Purity||> 97% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
Submit your question about RP00065 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.