Products > Catalog Proteins > Immune Checkpoint

Recombinant Human 4-1BB Ligand/TNFSF9 Protein (RP00060)

Datasheet

Overview

Product nameRecombinant Human 4-1BB Ligand/TNFSF9 Protein
Catalog No.RP00060
DescriptionRecombinant Human 4-1BB Ligand/TNFSF9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg71-Glu254) of human 4-1BB Ligand/TNFSF9 (Accession #NP_003802.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human (rh) 4-1BB/TNFRSF9/CD137 Fc Chimera is Immobilized at 10 ng/mL (100 μL/well), the concentration of rh4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.
SpeciesHuman
The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Synonym4-1BB-L; CD137L; TNLG5A
Expressed HostE. coli
Tag6×His tag at the C-terminus
Swiss portP41273
Gene ID8744
StorageStore the lyophilized protein at -20°C to -80 °C for long term. |After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Inquire About This Product

Submit your question about RP00060 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order