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Recombinant Human 4-1BB Ligand/TNFSF9 Protein (RP00060)



Product nameRecombinant Human 4-1BB Ligand/TNFSF9 Protein
Catalog No.RP00060
DescriptionRecombinant Human 4-1BB Ligand/TNFSF9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg71-Glu254) of human 4-1BB Ligand/TNFSF9 (Accession #NP_003802.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human (rh) 4-1BB/TNFRSF9/CD137 Fc Chimera is Immobilized at 10 ng/mL (100 μL/well), the concentration of rh4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.
The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Synonym4-1BB-L; CD137L; TNLG5A
Expressed HostE. coli
Tag6×His tag at the C-terminus
Swiss portP41273
Gene ID8744
StorageStore the lyophilized protein at -20°C to -80 °C for long term. |After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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