|Product name||Recombinant Human 4-1BB Ligand/TNFSF9 Protein|
|Description||Recombinant Human 4-1BB Ligand/TNFSF9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg71-Glu254) of human 4-1BB Ligand/TNFSF9 (Accession #NP_003802.1) fused with a 6×His tag at the C-terminus.|
|Bio-Activity||Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) 4-1BB/TNFRSF9/CD137 Fc Chimera is Immobilized at 10 ng/mL (100 μL/well), the concentration of rh4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.|
|Synonym||4-1BB-L; CD137L; TNLG5A|
|Expressed Host||E. coli|
|Tag||6×His tag at the C-terminus|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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