|Synonym||IL-27, IL-27A, IL27A, IL27p28, IL30, p28|
|Protein Construction||High quality, high purity and low endotoxin recombinant Human IL27 / Interleukin-27 Protein (His Tag) (10076-H08S), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.|
|Expressed Host||CHO Stable Cells|
|Shipping||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Purity||> 90 % as determined by SDS-PAGE|
|Bio-Activity||1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.2-0.46 μg/ml. 2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 23-92 ng/mL.|
|Endotoxin||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N Terminal||Arg 21|
|Molecule Mass||The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.|
|Storage||Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
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