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Active Recombinant Mouse IL-4 Protein (RP01161)

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Overview

Product nameActive Recombinant Mouse IL-4 Protein
Catalog No.RP01161
DescriptionActive Recombinant Mouse IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His23-Ser140) of mouse IL-4 (Accession #NP_067258.1) fused with a 6×His tag at the C-terminus.
Bio-Activity1.Measured by its ability to induce Mouse Bone marrow derived macrophage (BMDM) differentiation into M2-type. 20ng/mL of Recombinant Mouse IL-4 and Mouse IL-13 (RP01123) can effectively upregulate the expression of M2-type biomarker Arg-1.2.10 ng/mL of Mouse IL-4 and 20 ng/mL of Mouse GM-CSF induce C57BL/6J Mouse cells differentiate into BMDC. According to the Flow cytometry data, after 6 days, up to 75% of cells in the system expressed DC biomarker CD11C on cell surface, among which more than 15% cells have high expression of MHCII. These data shows that the induction was successful.3.Recombinant Mouse IL-4 protein (10 ng/mL) and Mouse GM-CSF protein (20 ng/mL) induce C57BL/6 Mouse bone marrow cells to differentiate into BMDC. After 7 days, up to 89.4% of cells in the system expressed DC biomarker CD11c on cell surface, among which more than half of the cells have high expression of CD80 and CD86 proteins. These data shows that the induction was successful.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesMouse
SynonymB-cell IgG differentiation factor; B-cell growth factor 1; BSF-1; Il-4; IGG1 induction factor; Lymphocyte stimulatory factor 1
Expressed HostHEK293 cells
TagC-His
Swiss-ProtP07750
Gene ID16189
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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