Product Type > Proteins

Recombinant Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Protein (RP00992)

Datasheet

Overview

Product name Recombinant Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Protein
Catalog No. RP00992
Description Active Recombinant Human TNFRSF10D/DcR2/TRAIL-R4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 56 - His 211 ) of human DcR2 (Accession #NP_003831.2) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TRAIL at 2 μg/mL (100 μL/well) can bind Recombinant Human DcR2 with a linear range of 15-60 ng/mL.
2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 1.8-7.2 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Purity > 95% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
Synonym CD264; DCR2; TRAIL-R4; TRAILR4; TRUNDD; TNFRSF10D; DCR2; TRAIL-R4; TRAILR4; TRUNDD
Expressed Host HEK293 cells
Tag C-hFc&His
Swiss-Prot Q9UBN6
Gene ID 8793
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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