Product Type > Proteins

Active Recombinant Human IL-2 Protein (RP01039)



Product name Active Recombinant Human IL-2 Protein
Catalog No. RP01039
Description Active Recombinant Human IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Thr153) of human IL2 (Accession #NP_000577.2) fused with a 6×His tag at the C-terminus.
Bio-Activity 1.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED50 for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 106~4.0× 106 units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.
Purity > 95% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Synonym IL2; IL-2; TCGF; lymphokine
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot P60568
Gene ID 3558
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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* For research use only. Not for therapeutic or diagnostic purposes.

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