|Product name||Active Recombinant Human IL-2 Protein|
|Description||Active Recombinant Human IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Thr153) of human IL2 (Accession #NP_000577.2) fused with a 6×His tag at the C-terminus.|
|Bio-Activity||1.Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 13.5-54 ng/mL.|
2.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED50 for this effect is 0.25-0.5 ng/mL.
3.Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 0.1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
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* For research use only. Not for therapeutic or diagnostic purposes.