| Product name | Active Recombinant Human IL-2 Protein |
|---|---|
| Catalog No. | RP01039 |
| Description | Active Recombinant Human IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Thr153) of human IL2 (Accession #NP_000577.2) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED50 for this effect is 0.25-0.5 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Human |
| Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
