Publications (3) Datasheet COA
Product name | Active Recombinant Human IFN-gamma Protein |
---|---|
Catalog No. | RP01038 |
Description | Recombinant Human Interferon Gamma Protein is produced by E. coli expression system. The target protein is expressed with sequence ( Gln24-Gln166) of human Interferon Gamma (Accession #NP_000610.2). |
Bio-Activity |
1.Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97 ng/mL. 2.Measured in a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 1.6-7.4 ng/mL, corresponding to a specific activity of 1.35×105~6.25×105 units/mg. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
---|---|
Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
Submit your question about RP01038 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Publishing research using RP01038? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.