Product Type > Proteins

Recombinant Human IFN-alpha 1/13(Q114A) Protein (RP00011)

Datasheet

Overview

Product name Recombinant Human IFN-alpha 1/13(Q114A) Protein
Catalog No. RP00011
Description Recombinant Human IFN-alpha 1/13(Q114A) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Cys24-Glu189 (Ala114)) of human IFNA1 (Accession #NP_076918.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
Bio-Activity Measured by its ability to stimulate GBP2 expression in 293T human embryonic kidney cells. 0.1-1 ng/mL of Recombinant Human IFNA1 can effectively Stimulating GBP2 expression.
2.Measured by its ability to stimulate human colon adenocarcinoma cells (Caco-2 cells). 10 ng/μL of Recombinant Human IFN-alpha 1/13(Q114A) can upregulate the expression of MDA5.
Purity > 97% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells.Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has has both anti-viral and immunomodulatory activities on target cells.
Synonym IFNA1; IFL; IFN; IFN-ALPHA; IFN-alphaD; IFNA13; IFNA
Expressed Host E. coli
Tag C-His
Swiss-Prot P01562
Gene ID 3439
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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